SJ000291942

SKU: orb1302673

Description

SJ000291942 is a small molecule activator of the canonical BMP signaling pathway. It is used in research to study bone formation, cellular differentiation, and embryogenesis in both in vitro cell-based assays and in vivo model systems.

Research Area

Stem Cell & Developmental Biology

Key Properties

• CAS Number: 425613-09-8
• MW: 318.3
• Purity: 99.66%
• Formula: C₁₆H₁₅FN₂O₄
• SMILES: CCc1ccc(OCC(=O)Nc2ccc(F)c(c2)[N+]([O-])=O)cc1
• Target: TGF-beta/Smad
• Solubility: DMSO:60 mg/mL (188.5 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (6.28 mM)

Bioactivity

• In Vitro:
  SJ000291942 is the most potent compound in causing severe ventralization in embryos, displaying higher mortality rates at lower doses compared to controls and two other compounds, indicating its strong induction of ventralization through enhanced BMP signaling activity. It elevates bmp2b and szl expression levels, suggesting activation of the canonical BMP signaling pathway in zebrafish assays. Further, immunoblotting experiments on C33A-2D2 cell lysates treated with SJ000291942 show its ability to activate SMAD1/5/8 phosphorylation within 1 hour in a serum-free medium, mirroring its high activity observed in zebrafish. Additionally, SJ000291942 significantly induces phosphorylation of Extracellular Signal-regulated Kinase, ERK1/2 (P-ERK1/2). High doses of BMP4 treatments (100 and 300ng) result in a gene expression profile similar to that of osteoblasts, while a low dose (10ng) of BMP4 closely matches the effects observed with 25μM of both compound 3 and SJ000291942 treatments.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, inhibit, Transforming growth factor beta receptors, TGF-β/Smad, TGF-β Receptor, TGFβ, SJ000291942, SJ-000291942, SJ 000291942, Smad, TGFb, TGF-b/Smad, TGFbeta, TGF-beta, TGFbeta/Smad

Compound Identifiers

PubChem CID

875819