SIRT1 antibody

• Catalog Number: orb420368
• Category: Antibodies
• Description: SIRT1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Affinity purified Anti-SIRT1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the c-terminal of human SIRT1 protein.
• Concentration: 1.07 mg/ml
• Dilution range: ELISA: 5.0 ug/ml, WB: 1.0 ug/ml
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-SIRT1 is directed against human SIRT1. This product is an affinity purified antibody produced by immunoaffinity chromatography. A BLAST analysis used to suggest reactivity with this protein from human, mouse, rat based on 100% homology for the immunizing sequence.
• Conjugation: Unconjugated
• UniProt ID: Q96EB6
• NCBI: NP_001135970.1
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-SIRT1 antibody, NAD-dependent protein
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~62 or 81.7 kDa in size corresponding to SIRT1 by western blotting in the appropriate cell lysate or extract. Higher observed band ~125 kda is due to complexing proteins.
• Expiration Date: 12 months from date of receipt.