SIRT1 Antibody

SKU: orb420368

Description

SIRT1 antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 5.0 ug/ml, WB: 1.0 ug/ml
• Reactivity: Human
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~62 or 81.7 kDa in size corresponding to SIRT1 by western blotting in the appropriate cell lysate or extract. Higher observed band ~125 kda is due to complexing proteins.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Affinity purified Anti-SIRT1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the c-terminal of human SIRT1 protein.
• Target: SIRT1
• Purity: Anti-SIRT1 is directed against human SIRT1. This product is an affinity purified antibody produced by immunoaffinity chromatography. A BLAST analysis used to suggest reactivity with this protein from human, mouse, rat based on 100% homology for the immunizing sequence.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.07 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-SIRT1 antibody, NAD-dependent protein deacetylase sirtuin-1, hSIRT1, Regulatory protein SIR2 homolog 1, SIR2-like protein 1, hSIR2, SirtT1 75 kDa fragment, 75SirT1, SIRT-1, SIRT 1, SIR2L1

Western Blot of Rabbit anti-SIRT1 antibody. Lane 1: MW ladder (opal pre-stained). Lane 2: HCT-116 WCL (p/n orb348712). Lane 3: K-562 WCL (p/n orb348692). Lane 4: Jurkat WCL (p/n orb348674). Lane 5: A549 WCL (p/n orb348675). Lane 6: LnCap WCL (p/n orb348694). Load: 35 µg per lane. Primary antibody: SIRT1 Antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary HRP antibody (p/n orb347654) at 1:70000 for 45 min at RT. Block: BlockOut (p/n orb348644) overnight at 4°C. Predicted size: 82 kDa, 61 kDa for SIRT1. Observed size: ~110 kDa due to complexing proteins.

Quick Database Links

Gene Symbol

SIRT1

UniProt

Q96EB6

RefSeq (Protein):NP_001135970.1