SIRT1 Antibody

SKU: orb420367

Description

SIRT1 antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 5.0 µg/ml, WB: 1.0 ug/ml
• Reactivity: Human
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~62 or 81.7 kDa in size corresponding to SIRT1 by western blotting in the appropriate cell lysate or extract. Higher observed band ~125 kda is due to complexing proteins.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Affinity purified Anti-SIRT1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the c-terminal of human SIRT1 protein.
• Target: SIRT1
• Purity: Anti-SIRT1 is directed against human SIRT1. This product is an affinity purified antibody produced by immunoaffinity chromatography. A BLAST analysis used to suggest reactivity with this protein from human, mouse, rat based on 100% homology for the immunizing sequence.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.07 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-SIRT1 antibody, NAD-dependent protein deacetylase sirtuin-1, hSIRT1, Regulatory protein SIR2 homolog 1, SIR2-like protein 1, hSIR2, SirtT1 75 kDa fragment, 75SirT1, SIRT-1, SIRT 1, SIR2L1

Western Blot of Rabbit anti-SIRT1 antibody. Lane 1: MW ladder (opal pre-stained). Lane 2: HCT-116 WCL (p/n orb348712). Lane 3: K-562 WCL (p/n orb348692). Lane 4: Jurkat WCL (p/n orb348674). Lane 5: A549 WCL (p/n orb348675). Lane 6: LnCap WCL (p/n orb348694). Load: 35 µg per lane. Primary antibody: SIRT1 Antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary HRP antibody (p/n orb347654) at 1:70000 for 45 min at RT. Block: BlockOut (p/n orb348644) overnight at 4°C. Predicted size: 82 kDa, 61 kDa for SIRT1. Observed size: ~110 kDa due to complexing proteins.

Quick Database Links

Gene Symbol

SIRT1

UniProt

Q96EB6

RefSeq (Protein):NP_001135970.1