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| Catalog Number | orb781900 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Luteinizing Hormone(LH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Luteinizing Hormone(LH). |
| Tested applications | ELISA |
| Reactivity | Primate |
| Range | 468.75-30000 pg/mL |
| Assay Time | 3.5h |
| Concentration | 100 mIU/mL |
| Sensitivity | 172.5 pg/mL |
| Alternative names | ICSH ELISA Kit, Lutropin ELISA Kit, Interstitial C Read more... |
| Note | For research use only |
| Application notes | standard: 100 mIU/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian LH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian LH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian LH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian LH in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | Serum, plasma and other biological fluids. |
| Expiration Date | Please enquire. |
