Simian CD276(Cluster Of Differentiation 276) ELISA Kit

SKU: orb3127110

Description

Simian CD276(Cluster Of Differentiation 276) ELISA Kit

Research Area

Immunology & Inflammation

Images & Validation

• Application Notes:
  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian CD276. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian CD276. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian CD276, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian CD276 in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

• Reactivity: Primate
• Sample Types: serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.117 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

B7-H3; B7H3; CD276 Molecule; B7 homolog 3; Costimulatory molecule