SIGLEC15 Antibody

• Catalog Number: orb1539217
• Category: Antibodies
• Description: SIGLEC15 Antibody
• Target: SIGLEC15
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS containing 0.02% Sodium Azide. PBS containing 0.02% Sodium Azide
• Purification: Peptide Column Affinity Chromatography
• Immunogen: A 15 AA synthetic peptide near the N-Terminus of human SIGLEC15. The immunogen is located within aa 50-100 of SIGLEC15.
• Tested applications: ELISA, IHC, IHC-P, WB
• Dilution range: ELISA, IHC, IHC-P (5 µg/ml), WB (1 - 2 µg/ml)
• Application notes: Further information: SIGLEC15 antibody can be used for detection of SIGLEC15 by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Western Blot: Predicted: 36 kDa Observed: 42 kDa
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SIGLEC15, CD33 antigen-like 3, CD33L3, HsT1361, SI
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Loading: 15 µg of lysates per lane. Antibodies: SIGLEC15, (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.