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PTPN6 Antibody

SKU: orb1247553

Description

PTPN6 Antibody

Research Area

Signal Transduction

Images & Validation

Tested ApplicationsELISA, IHC, WB
ReactivityHuman, Mouse, Rat
Predicted ReactivityCanine
Application Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 65kDa band observed in Human Bone Marrow and Liver lysates and in Mouse and Rat Spleen and Thymus lysates (calculated MW of 67.6kDa according to NP_536858.1and NP_002822.2). Recommended concentration: 0.1-0.3µg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Thyroid. Recommended concentration: 5µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-KASRTSSKHKEE
TargetPTPN6
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

PTPN6, SHP1, protein tyrosine phosphatase, non-receptor type 6 , HCP, HCPH, HPTP1C, PTP-1C, SH-PTP1, SHP-1, SHP-1L, 70 kda SHP-1L protein, hematopoietic cell phosphatase, hematopoietic cell protein-tyrosine phosphatase, protein-tyrosine phosphatase 1C

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PTPN6 Antibody

orb1247553 (0.1 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

PTPN6 Antibody

orb1247553 (5 ug/ml) staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

PTPN6 Antibody (orb1247553)

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0.1 mg
$ 920.00
Free Secondary Antibody (20 ul)0/0

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