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| Catalog Number | orb866921 |
|---|---|
| Category | Assays and Kits |
| Description | Sandwich Sheep SHBG ELISA Kit |
| Reactivity | Sheep |
| Concentration | 100 ng/mL |
| Tested applications | ELISA |
| Application notes | standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep SHBG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep SHBG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep SHBG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep SHBG in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Time | 3.5h |
| Range | 1.57-100 ng/mL |
| Sensitivity | 0.59 ng/mL |
| Sample Types | serum, plasma and other biological fluids |
| Alternative names | ABP ELISA Kit, TEBG ELISA Kit, SBP ELISA Kit, Sex Read more... |
| Note | For research use only |
