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| Catalog Number | orb1146763 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nitric Oxide Synthase 3, Endothelial(NOS3). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Nitric Oxide Synthase 3, Endothelial(NOS3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nitric Oxide Synthase 3, Endothelial(NOS3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nitric Oxide Synthase 3, Endothelial(NOS3) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Sheep |
| Range | 15.63-1000 pg/mL |
| Assay Time | 3.5h |
| Concentration | 1000 pg/mL |
| UniProt ID | P79209 |
| Sensitivity | 5.8 pg/mL |
| Alternative names | ECNOS, NOS III, E-NOS, C-NOS, ENOS, CNOS, Constitu Read more... |
| Note | For research use only |
| Application notes | standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep NOS3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep NOS3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep NOS3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep NOS3 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | Serum, plasma, tissue homogenates, cell lysates and other biological fluids. |
| Expiration Date | Please enquire. |
