Sheep Neurokinin B (NKB) ELISA Kit

• Catalog Number: orb1173510
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Neurokinin B(NKB) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Neurokinin B(NKB). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neurokinin B(NKB) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Tested applications: ELISA
• Reactivity: Sheep
• Range: 12.5-800 pg/mL
• Assay Time: 2.5h
• Concentration: 800 pg/mL
• Sensitivity: 3.25 pg/mL
• Alternative names: NK-B, TAC3, NKNB, ZNEUROK1, Neuromedin K, Tachykin
• Note: For research use only
• Application notes: standard: 800 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Sheep NKB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep NKB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep NKB in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: Serum, plasma and other biological fluids.
• Expiration Date: Please enquire.