Sheep Insulin Like Growth Factor 1 (IGF1) ELISA Kit

SKU: orb2667379

Description

Sheep Insulin Like Growth Factor 1 (IGF1) ELISA Kit

Images & Validation

• Application Notes:
  standard: 1000 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep IGF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep IGF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep IGF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep IGF1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 15.63-1000 ng/mL
• Sensitivity: 6.1 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

IGFI; IGF1A; IBP1; MGF; Somatomedin C; Mechano Growth Gactor

Quick Database Links

UniProt

P10763