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| Catalog Number | orb2667380 |
|---|---|
| Category | Assays and Kits |
| Description | Sheep Estradiol (E2) ELISA Kit |
| Application notes | standard: 3000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Sheep E2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep E2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep E2 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Time | 2.5h |
| Range | 46.88-3000 pg/mL |
| Sensitivity | 13.69 pg/mL |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Alternative names | 17B-Estradiol; Oestradiol; Beta-Estradiol |
| Note | For research use only |
Sheep Estradiol ELISA Kit [orb340130]
Sheep
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10 x 96 T, 24 T, 5 x 96 T, 48 T, 96 T
