Sheep Rabbit IgG (H&L), TRITC conjugated Antibody

SKU: orb700251

Description

Sheep polyclonal antibody against Sheep anti-Rabbit IgG (H&L) conjugated to TRITC

Images & Validation

• Dilution Range: The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
• Reactivity: Rabbit
• Application Notes:
  Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins

Key Properties

• Host: Sheep
• Clonality: Polyclonal
• Immunogen: Purified rabbit IgG (H&L)
• Purification: Immunogen affinity purified sheep IgG.
• Conjugation: TRITC

Storage & Handling

• Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
• Form/Appearance: Lyophilized
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only