SH-4-54

SKU: orb1300572

Description

SH-4-54

Research Area

Signal Transduction, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 1456632-40-8
• MW: 610.59
• Purity: 98.00%
• Formula: C₂₉H₂₇F₅N₂O₅S
• SMILES: CN(CC(=O)N(Cc1ccc(cc1)C1CCCCC1)c1ccc(cc1)C(O)=O)S(=O)(=O)c1c(F)c(F)c(F)c(F)c1F
• Target: STAT
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:3.3 mg/mL (5.4 mM);DMSO:93 mg/mL (152.31 mM);H2O:< 1 mg/mL (insoluble or slightly soluble);Ethanol:47 mg/mL (76.97 mM)

Bioactivity

• Target IC50:
  STAT3:300 nM(Kd)|STAT5:464 nM(Kd)
• In Vivo:
  Intraperitoneal injection of 10 mg/kg SH-4-54 into mice with orthotopic xenografts of BT73 effectively inhibite the growth of glioma and suppressed pSTAT3 activation.
• In Vitro:
  SH-4-54 effectively inhibit the phosphorylation of STAT3 and its downstream transcription targets. Additionally, SH-4-54 demonstrates unparalleled cytotoxicity against human malignant gliomas and liver cancer cells while showing no toxicity toward human embryonic astrocytes.
• Cell Research:
  BTSC spheres are dissociated to Single cells wit the enzyme Accumax, seeded at 1500 cells/ 96-well and treated with drug or Vehicle (DMSO) one day after plating. Cytotoxicity studies are repeated independently using BTSC lines 25M, 67EF, 73EF, 84EF and 127EF. BTSC spheres are dissociated to Single cells as above and plated in 96 well plates in triplicate at 3000 cells/ 96-well. In both sets of experiments drugs are used as serial dilutions withi the range of 5 μM to 100 nM in the first set and 25 μM to 10 nM. Cell viability following drug treatment is assessed three days later usin the alamarBlue assay according to the manufacturer's instructions. All culture experiments are performed in triplicate with a minimum of three wells per condition.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

STAT, STAT5, STAT3, SH454, SH-4-54, SH 4 54, Inhibitor, inhibit

Compound Identifiers

PubChem CID

72188643