SF1670

SKU: orb1300574

Description

SF1670 is a cell-permeable small molecule inhibitor of the phosphatase PTEN, exhibiting an IC50 of 2 μM. It is utilized in biochemical and cellular research to investigate PTEN-related signaling pathways in oncology and immunology, facilitating studies on cell growth and survival mechanisms.

Research Area

Cell Biology, Metabolism Research, Signal Transduction

Key Properties

• CAS Number: 345630-40-2
• MW: 307.34
• Purity: 98.54% (May vary between batches)
• Formula: C₁₉H₁₇NO₃
• SMILES: CC(C)(C)C(=O)Nc1ccc-2c(c1)C(=O)C(=O)c1ccccc-21
• Target: PTEN,Phosphatase,Autophagy
• Solubility: 10% DMSO+90% Corn Oil:2 mg/mL (6.51 mM);DMSO:30.7 mg/mL (99.89 mM)

Bioactivity

• Target IC50:
  PTEN:2 μM
• In Vivo:
  Pretreatment of SF1670 (500 nM i.v.) augments bacteria-killing capability in neutropenic mice in both peritonitis and bacterial pneumonia, and decreases the mortality of neutropenia-related pneumonia.
• In Vitro:
  SF1670 shows potent cytotoxicity in HBEC, PC-3, H1299 cells with IC50 of 5 μM, 10 μM, 44 μM, respectively. In a mouse aortic ring matrigel angiogenesis model, SF1670 stimulates the angiogenic processes. SF1670 augments chemoattractant-elicited PtdIns(3,4,5)P3 signaling in neutrophils and enhances neutrophil functions.
• Cell Research:
  Cells are plated into 96-well plates in RPMI 1640 medium supplemented with 10% FBS and incubated overnight at 37°C. in an incubator containing an atmosphere of 5% CO2. The following day, the medium is replaced and cells are starved by placing in 100 uL of serum-free medium for 3 hours. Serially diluted test compounds are added to the wells and incubated with the cells for 2 hours at 37°C. Compounds are tested in a range from 1 mM to 0.1 nM depending on solubility. MTT is added to the wells at a final concentration of 5 μg/ml and incubated with the cells for 3 more hours. At the end of the incubation, the medium is aspirated and the MTT stain in the cells is dissolved by the addition of 100 μL DMSO. Optical density of each well is then measured at 570 nm using a SpectroMax Plus spectrophotometric plate reader. The IC50 determination from the data was made using Prism software.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, inhibit, MMAC1, Autophagy, SF1670, SF-1670, Phosphatase and tensin homolog, Phosphatase, SF 1670, PTEN, PTP CD45 Inhibitor, PTPase CD45 Inhibitor

Compound Identifiers

PubChem CID

9926586