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Description
Research Area
Cancer Biology, Cell Biology, Immunology & Inflammation, Metabolism Research, Signal Transduction
Images & Validation
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Item 1 of 1
| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 in 100μl reaction mixture containing 1. 25 ng trypsin, 10μM substrate, 50 mM Tris, 10 mM CaCl2, 0. 15 M NaCl , pH 7. 5. The IC50 value is approximately <4. 5 nM. |
| Target | SERPINA1 |
| Tag | His Tag |
| MW | 45.1 kDa |
| Purity | >95% as determined by SDS-PAGE. |
| Protein Sequence | Glu 25 - Lys 418 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−SerpinA1, PI, A1A, AAT, PI1, A1AT, MGC9222, PRO2275, MGC23330，alpha1AT, SPAAT
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Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
Available Sizes
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