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Description
Research Area
Images & Validation
−| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 in 100μl reaction mixture containing 1. 25 ng trypsin, 10μM substrate, 50 mM Tris, 10 mM CaCl2, 0. 15 M NaCl , pH 7. 5. The IC50 value is approximately <4. 5 nM. |
| Target | SERPINA1 |
| Tag | His Tag |
| MW | 45.1 kDa |
| Purity | >95% as determined by SDS-PAGE. |
| Protein Sequence | Glu 25 - Lys 418 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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Human Serpin A1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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Protocol Information
Serpin A1 Recombinant Protein (orb1227882)
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