SDS-PAGE Protein Loading Buffer 5X (Reducing)

SKU: orb90545

Description

SDS-PAGE Protein Loading Buffer is a ready-to-use 5X solution. It contains 10% SDS, 500Mm DTT, 50% Glycerol, 500mM Tris-HCL and 0.05% bromophenol blue dye. It can be used for SDS-PAGE protein loading of conventional proteins. It is especially formulated for protein sample preparation to be used in the Laemmli SDS-PAGE system. It is sufficient to prepare 15 mL protein samples. The whole sample preparation process can be finished within 5 minutes.

Images & Validation

• Application Notes:
  Assay Protocol 1. Equilibrate SDS-PAGE Protein Loading Buffer 5X to room temperature or thaw Loading Buffer in a water bath no higher than 30°C. 2. Mix one volume of SDS-PAGE Protein Loading Buffer 5X with four volume of protein sample (i.e. add 4mL protein sample into 1 mL Loading Buffer). 3. Boil sample for 3-5 min. Note: If semitransparent viscous substance remains after boiling, boil sample for another 5-10 min or add 1X SDS-PAGE Protein Loading Buffer which is diluted from 5X SDS-PAGE Protein Loading Buffer to the sample and then boil for another 3-5 min. 4. Allow sample to cool to room temperature. 5. Load sample into the wells of the SDS-PAGE gel and begin electrophoresis. 6. Stop electrophoresis when bromophenol blue dye front reaches to the bottom of the gel. General Notes 1. SDS-PAGE Protein Loading Buffer 5X contains DTT which has a slightly irritating odor. 2. SDS-PAGE Protein Loading Buffer 5X contains DTT which has a slightly irritating odor. 3. Please wear the lab coat and disposable gloves and operate in a laboratory hood.

Storage & Handling

• Storage: Upon receipt store at -20°C. SDS-PAGE Protein Loading Buffer 5X (Reducing) is stable for one year. Product is shipped on ice.
• Form/Appearance: Liquid
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Li-Juan Nie 1 2, Zhe Cheng 1, Yi-Xian He 1, Qian-Hua Yan 1, Yao-Huan Sun 1, Xin-Yi Yang 1, Jie Tian 1, Peng-Fei Zhu 1, Jiang-Yi Yu 1, Hui-Ping Zhou 3, Xi-Qiao Zhou Role of duodenal mucosal resurfacing in controlling diabetes in rats World J Diabetes, (2025)

PubMed: 40093272

Applications

WB

Reactivity

Rat