SCRIPT Reverse Transcriptase

• Catalog Number: orb533398
• Category: Tools
• Description: SCRIPT Reverse Transcriptase is a genetically engineered version of M-MLV Reverse Transcriptase (M-MLV RT) with eliminated RNase H activity and increased thermal stability. The enzyme is a RNA-directed DNA polymerase that synthesizes a complementary DNA strand initiating from a primer using single-stranded RNA or DNA as template. Its enhanced thermal stability in combination with the deactivated RNase H activity results in an increased speci-ficity, higher cDNA yield and an improved efficiency for full length cDNA synthesis compared with standard M-MLV RT. The enzyme is recommended for synthesis of cDNA from 100 bp up to 10 kb length.
• Concentration: 200 units/µl
• Form/Appearance: liquid
• Purity: free of endo-and exodeoxyribonucleases, phosphatases and ribonuclease
• Storage: store at -20 °C
• Note: For research use only
• Application notes: Extremely sensitive and highly specific RT-PCR, synthesis of highly structured and long cDNA fragments, DNA labelling.
• Expiration Date: 6 months from date of receipt.