SCRIPT Reverse Transcriptase

SKU: orb533398

Description

SCRIPT Reverse Transcriptase is a genetically engineered version of M-MLV Reverse Transcriptase (M-MLV RT) with eliminated RNase H activity and increased thermal stability. The enzyme is a RNA-directed DNA polymerase that synthesizes a complementary DNA strand initiating from a primer using single-stranded RNA or DNA as template. Its enhanced thermal stability in combination with the deactivated RNase H activity results in an increased speci-ficity, higher cDNA yield and an improved efficiency for full length cDNA synthesis compared with standard M-MLV RT. The enzyme is recommended for synthesis of cDNA from 100 bp up to 10 kb length.

Images & Validation

• Application Notes:
  Extremely sensitive and highly specific RT-PCR, synthesis of highly structured and long cDNA fragments, DNA labelling.

Key Properties

• Purity: free of endo-and exodeoxyribonucleases, phosphatases and ribonuclease

Storage & Handling

• Storage: store at -20 °C
• Form/Appearance: liquid
• Concentration: 200 units/µl
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Reverse Transcriptase with increased thermal stability