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SCRIPT Direct RT-qPCR GreenMaster highROX
Catalog Number: orb1733646
| Catalog Number | orb1733646 |
|---|---|
| Category | Small Molecules |
| Description | SCRIPT Direct RT-qPCR SybrMaster highROX is designed for quan-titative real-time analysis of target RNA directly from animal-or plant tissue, swabs and blood. The mix allows robust amplification avoiding the requirement of any prior RNA purification procedures. The mix contains SYBR Green Fluorescent DNA Stain. It allows fast and easy quantification of sample RNA over a wide dynamic range with exceptional sensitivity and precision. The mix contains all reagents required for RT-qPCR (except template and primers) in a premixed 2x concentrated ready-to-use solution. High robustness, reliability and sensitivity of the mix are based on a genetically engineered reverse transcriptase and an antibody-blocked hot start polymerase in combination with an optimized and well-balanced buffer system. The mix ensures fast and easy preparation with a minimum of pipetting steps and is specially recommended for: Direct amplification of target RNA from various tissues samples; Direct detection of viral or bacterial RNA in nasal or throat swabs; Direct PCR from whole samples; Point-of-Care diagnostics. |
| Form/Appearance | liquid |
| Concentration | 2x |
| Storage | store at -20 °C. avoid freeze/thaw cycles, store dark. stable at 4 °C for up to 4 weeks |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
For example, if the dosage of your experiment is 10mg/kg, the weight of each animal is 50g, and the volume of the dosing is 100μL, then the working solution concentration of your experiment is 5mg/mL. If the formula you used is 5% DMSO+ 30% PEG300+ 5% Tween 80 + 60% Saline/PBS/ddH2O, the preparation method is as follows:
Mother liquor preparation method: 5mg of drug dissolved in 100μL DMSO (mother liquor concentration is 50mg/mL). Preparation method of in vivo formulation: For 1mL of working solution preparation, take 50μL of DMSO mother liquor, add 300 μL of PEG300 to mix well and clarify, then add 50μL of Tween-80 to mix well and clarify, and finally add 600μL of Saline/PBS/ddH2O to mix well and clarify.Note:
- For Reference Only. Please choose the appropriate dissolution protocol according to your experimental animal and administration method.
- We suggest that in cell experiments, the final concentration of DMSO is not more than 0.1%, in animal experiments, for healthy mice, the final concentration of DMSO is not more than 10%; For nude or frail mice, the final concentration of DMSO is not more than 2%.
