SCR130

SKU: orb1218325

Description

1,7,9-Triazaspiro[4.5]dec-1-ene-6,10-dione, 2,4-bis(4-chlorophenyl)-8-thioxo- is enzyme inhibition, antiproliferative effects and antifungal activity.

Key Properties

• CAS Number: 2377858-38-1
• MW: 418.3
• Purity: >98% (HPLC)
• Formula: C₁₉ H₁₃ Cl₂ N₃ O₂ S
• SMILES: O=C(NC(NC1=O)=S)C21C(C3=CC=C(Cl)C=C3)CC(C4=CC=C(Cl)C=C4)=N2
• Target: Others
• Solubility: In Vitro: DMSO : 100 mg/mL (239.06 mM)

Bioactivity

• In Vitro:
  SCR130 (7-21 μM; 48 hours) increase in the number of late and early apoptotic cells. SCR130 induces apoptosis by both intrinsic and extrinsic pathways. SCR130 increases the expression of p-p53, BCL2 and MCL1, and CYTOCHROME C, BAX, and BAK also increaseed. The activation of caspase 8, increase in expression of FAS and SMAC-DIABLO proteins are also observed. SCR130 (48 hours) exhibits cytotoxicity in Reh, HeLa, CEM, Nalm6, and N114 cells with IC50 values of 14.1 μM, 5.9 μM, 6.5 μM, 2.2 μM, and 11 μM, respectively. SCR130 can potentiate the effect of radiation (0.5 and 1 Gy) by inducing enhanced cell death upon coadministration in Reh and Nalm6 cell lines. SCR130 blocks the endogenous NHEJ leading to accumulation of unrepaired DNA breaks. Treatment with SCR130 leads to inhibition of endogenous NHEJ, resulting in the accumulation of DNA double-strand breaks (DSBs) and cell death by activating apoptotic pathways.: Apoptosis Analysis Cell line: Reh cells. Concentration: 7 μM, 14 μM, and 21 μM. Incubation time: 48 hours. Result: Showed a concentration-dependent increase in the number of late and early apoptotic cells. Western blot analysis. Cell line: Reh cells. Concentration: 7 μM, 14 μM, and 21 μM. Incubation time: 48 hours. Result: Revealed a concentration-dependent increase in levels of pATM and activation of p53 through phosphorylation.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

1,7,9-Triazaspiro[4.5]dec-1-ene-6,10-dione, 2,4-bis(4-chlorophenyl)-8-thioxo-

Quick Database Links

Gene Symbol

Others