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| Catalog Number | orb1972246 |
|---|---|
| Category | Assays and Kits |
| Description | Scicons Double-stranded RNA (dsRNA) ELISA kit (J2 based) |
| Clonality | Monoclonal |
| Species/Host | Mouse |
| Isotype | IgG2a kappa/IgM kappa |
| Tested applications | ELISA |
| Application notes | We recommend using the kit to detect viral dsRNAs or large natural or synthetic dsRNAs of non-viral origin in nucleic acid extracts, as well as to detect the presence of undesired dsRNA molecules in artificially synthesized (m)RNA preparations. Serial dilutions of the Poly(I:C) dsRNA standard (included in the kit) can be used as a positive control. For the exact detection protocol we refer to the kit manual that can be downloaded from our website. Caution: The Poly(I:C) dsRNA positive control included in this sandwich ELISA kit is not intended to be used as a quantitative standard for other dsRNA preparations. The anti dsRNA antibodies J2 and/or K2 used in this kit may exhibit a different degree of reactivity with different dsRNAs obtained from synthetic or natural sources. It is therefore only intended to be used as a positive control to see if the ELISA has been executed correctly and that the test shows a linear relationship between the amount of dsRNA and the read out for the OD450. |
| Source | The two dsRNA antibodies used in this kit were produced as follows: Female DBA/2 mice were injected intraperitonially with a mixture of 50 ug L-dsRNA and 75 ug methylated bovine serum albumin, emulsified in complete Freund's adjuvant. After several boosts spleen cells were fused with Sp2/0-Agl4 myeloma cells to generate the hybridoma clones J2 and K2. |
| Storage | Upon receipt, store entire kit at -20°C. Once the kit is thawed, you may keep it at 4°C for 5 days. For long-term storage, it is recommended to aliquot and freeze the antibody and dsRNA components at –20°C. |
| Alternative names | dsRNA ELISA kit |
| Note | For research use only |
