SARS-CoV2-2 Iota Variant Recombinant Spike Timer His Tag

• Catalog Number: orb1945949
• Category: Proteins
• Description: SARS-CoV2-2 Iota Variant Recombinant Spike Timer His Tag
• Tested applications: ELISA
• Reactivity: Other
• Tag: His tag
• Isotype: SARS-CoV-2
• Concentration: 0.5 mg/ml
• Dilution range: Control antigen for SARS-CoV-2 (COVID-19) diagnostic assays. Contains C-terminal His tag used for purification.
• Storage: 2-8°C
• Buffer/Preservatives: 1X PBS, 0.025% NaN3
• Alternative names: Control antigen for SARS-CoV-2 (COVID-19) diagnost
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

Concentration-response curves for binding of CoV2 spike protein Iota variant to human ACE2 in cell-free ELISA-type assays. Microtiter wells were coated with 100 uL of each spike trimer at 2 ug/mL in PBS at 4?C overnight. The wells were washed with PBS and blocked with 200 μL of 1% BSA/PBS. ACE2-Fc was serially diluted from 2 μg/mL in 1% BSA/PBS. The blocker was discarded, and the wells were incubated with 100 μL of serially diluted ACE2-Fc at 37?C for 1 hour. The wells were washed with PBS and the bound ACE2-Fc was detected with 100 μL of Peroxidase AffiniPure Goat Anti-Human IgG, Fc? fragment specific (1:5, 000 in 1% BSA/PBS) at 37?C for 1 hour. The wells were washed with PBS and the wells were developed with 100 μL of MB/E Ultra Sensitive, Blue, Horseradish Peroxidase Substrate at RT for 5 min. The reaction was stopped with 100 μL of 0.6N H2SO4 and the signals were read at 450 nm using a plate reader.