SARS-CoV-2 Mpro-IN-29 is a potent main protease (Mpro) inhibitor with an IC50 of 310 nM, blocking viral polyprotein processing. It demonstrates antiviral activity in Vero cells (EC50 = 0.5 μM) and is a valuable tool compound for in vitro antiviral research and drug development against SARS-CoV-2.