SARS CoV-2 full length spike protein in DIBMA Glycerol

• Catalog Number: orb1231039
• Category: Proteins
• Description: SARS CoV-2 full length spike protein in DIBMA Glycerol
• Tested applications: ELISA
• Tag: C-terminal Rho1D4
• Concentration: batch dependent
• Dilution range: This recombinant protein can be used for ELISA assays, Ligand binding assays (e.g. SPR), Biochemical and biophysical analyses. For research use only.
• Form/Appearance: Liquid
• Purity: > 98% as determined by SDS-PAGE, see Fig. 1 A and B. PureCube Rho1D4 Agarose.
• MW: 1286 amino acids (including Rho1D4 tag and linker); 142114 Da
• Target: S
• UniProt ID: P0DTC2
• Protein Sequence: Full-length sequence (aa 1 – 1273),furin cleavage site “RRAR” mutated to “GSAG”;C-terminal Rho1D4 tag fused with spacer “GSSG” to protein sequence
• Source: HEK293 cells
• Biological Activity: not determined
• Storage: –80°C. Avoid freeze-thaw cycles
• Buffer/Preservatives: 20 mM Hepes pH 7.5; 150 mM NaCl,: buffer does not contain free DIBMA Glycerol (DIBMA: Diisobutylene-maleic acid)
• Alternative names: SARS-CoV-2 (COVID-19) Spike Recombinant Protein: S
• Note: For research use only
• Application notes: This recombinant protein can be used for ELISA assays, Ligand binding assays (e.g. SPR), Biochemical and biophysical analyses. For research use only.
• Expiration Date: 6 months from date of receipt.

A: SDS of SPIKE in synthetic nanodisc. B: Western blot of Rhot1d4 tagged SPIKE, originated from the SDS Page seen in "A". C: Native protein Gel of purified and stabilized SPIKE in synthetic nanodisc.