SARS-CoV-2 (COVID-19) Spike (D614G) (Stable Trimer) Recombinant Protein

• Catalog Number: orb1231041
• Category: Proteins
• Description: SARS-CoV-2 (COVID-19) Spike (D614G) (Stable Trimer) Recombinant Protein
• Concentration: 0.2mg/ml after reconstitution.
• Form/Appearance: Lyophilized
• Purity: ≥90% (SDS-PAGE)
• MW: ~190kDa (SDS-PAGE)
• Target: S
• UniProt ID: P0DTC2
• Protein Sequence: Full-length soluble SARS-CoV-2 Spike protein (aa 16-1213) including a foldon trimerization motif, mutated Furin recognition site (R682S, R685S) and two stabilizing mutations (K986P and V987P) is fused at the C-terminus to a His-tag.
• Source: HEK293 cells
• Biological Activity: Binds to the human SARS-CoV-2 receptor ACE2. Binds to anti-SARS-CoV-2 Spike antibodies in serum or plasma.
• NCBI: P0DTC2
• Storage: Short Term StorageL: +4°C, Long Term Storage: -20°C, Handling Advice: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. For maximum product recovery after thawing, centrifuge the vial before opening the cap. Use/Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
• Buffer/Preservatives: Lyophilized. Contains PBS and 0.5% trehalose.
• Alternative names: 2019-nCoV Spike Protein S
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

Full-length trimeric SARS-CoV-2 Spike binds to its receptor ACE2 (human) (rec.) with high affinity.

SDS-PAGE: Coomassie staining under non-reducing conditions, showing one clear band at 190kDa and confirming purity of the protein.

SARS-CoV-2 Spike Protein (D614G) (Stable Trimer) (rec.) (His) migrates by Size Exclusion Chromatography (SEC) as a single peak.