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Catalog Number | orb1231603 |
---|---|
Category | Proteins |
Description | SARS-CoV-2 (COVID-19) S1+S2 ECD (S-ECD) Recombinant Protein |
Tested applications | ELISA, WB |
Tag | His tag at C-terminus |
Form/Appearance | Lyophilized |
Purity | > 97% as determined by SDS-PAGE. |
MW | 132.29a |
Target | S |
UniProt ID | P0DTC2 |
Protein Sequence | Cys15-Gln1208 with mutation in the cleavage site (683 G) |
Source | HEK293 cells |
Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-COV-2 Spike S1+S2 ECD at 2μg/mL (100 μL/well) can bind Recombinant Human ACE2 with a linear range of 0.15-3.03 ng/mL |
NCBI | QHD43416.1 |
Storage | This product is stable at ≤ ‑70˚°C for up to 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | PBS, pH 7.4 |
Alternative names | 2019-nCoV spike protein, 2019-nCoV S glycoprotein, Read more... |
Note | For research use only |
Application notes | This recombinant protein can be used for WB. For research use only. |
Expiration Date | 6 months from date of receipt. |
Immobilized SARS-CoV-2 (COVID-19) S1 + S2 ECD (S-ECD) recombinant protein at 2μg/mL (100 μL/well) can bind recombinant human ACE2 with a linear range of 1.5-35.1 ng/mL.
SARS-CoV-2 (COVID-19) S1 + S2 ECD (S-ECD) recombinant protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 170-220 kDa.
WB | |
> 95% by SDS-PAGE. | |
HEK293 cells |
ELISA, WB | |
> 97% as determined by SDS-PAGE. | |
133.8 kDa | |
HEK293 cells |