SARS-CoV-2 (COVID-19) S1+S2 ECD (S-ECD) Recombinant Protein

• Catalog Number: orb1231603
• Category: Proteins
• Description: SARS-CoV-2 (COVID-19) S1+S2 ECD (S-ECD) Recombinant Protein
• Tested applications: ELISA, WB
• Tag: His tag at C-terminus
• Dilution range: This recombinant protein can be used for WB. For research use only.
• Form/Appearance: Lyophilized
• Purity: > 97% as determined by SDS-PAGE.
• MW: 132.29a
• Target: S
• UniProt ID: P0DTC2
• Protein Sequence: Cys15-Gln1208 with mutation in the cleavage site (683 G)
• Source: HEK293 cells
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-COV-2 Spike S1+S2 ECD at 2μg/mL (100 μL/well) can bind Recombinant Human ACE2 with a linear range of 0.15-3.03 ng/mL
• NCBI: QHD43416.1
• Storage: This product is stable at ≤ ‑70°C for up to 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.4
• Alternative names: 2019-nCoV spike protein, 2019-nCoV S glycoprotein,
• Note: For research use only
• Application notes: This recombinant protein can be used for WB. For research use only.
• Expiration Date: 6 months from date of receipt.

Immobilized SARS-CoV-2 (COVID-19) S1 + S2 ECD (S-ECD) recombinant protein at 2μg/mL (100 μL/well) can bind recombinant human ACE2 with a linear range of 1.5-35.1 ng/mL.

SARS-CoV-2 (COVID-19) S1 + S2 ECD (S-ECD) recombinant protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 170-220 kDa.