S100 Protein antibody

• Catalog Number: orb344628
• Category: Antibodies
• Description: S100 Protein antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Bovine, Human, Mouse, Rat
• Isotype: IgG
• Immunogen: This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full-length bovine S100 protein (mixture of aa homodimers and ab heterodimers).
• Concentration: 5.0 mg/mL
• Dilution range: ELISA: 1:5,000 - 1:25,000, IHC: 1:200 - 1:2,000, WB: 1:500 - 1:3,000
• Form/Appearance: Lyophilized
• Purity: This Protein A purified antibody is directed against bovine S100 protein. The product was purified from monospecific antiserum by Protein A chromatography. A BLAST analysis was used to suggest reactivity with this protein from bovine based on 100% homology with the immunogen sequence. A 98% homology is noted for S100 alpha chain from primate sources. Mouse, rat and dog show 94% homology with the bovine S100 alpha sequence. Expect cross reactivity with S100 from most mammalian sources. Cross reactivity with S100 from other specific sources has not been determined. Homologies for the S100 beta chain are similar.
• Conjugation: Unconjugated
• UniProt ID: P02639
• NCBI: NP_001092512.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-S100 Protein Antibody, S100 calcium-bi
• Note: For research use only
• Application notes: This Protein A purified antibody has been tested for use in immunohistochemistry, and western blot and is suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 11 kDa in size corresponding to S100 monomer by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of human brain astrocyte tumor tissue using S100 Protein antibody

Western blot analysis of rat brain whole cell lysate using S100 Protein antibody

Immunohistochemical staining of mouse brain tissue using S100 Protein antibody