S100A1 Antibody

SKU: orb344628

Description

S100 Protein antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:5,000 - 1:25,000, IHC: 1:200 - 1:2,000, WB: 1:500 - 1:3,000
• Reactivity: Bovine, Human, Mouse, Rat
• Application Notes:
  This Protein A purified antibody has been tested for use in immunohistochemistry, and western blot and is suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 11 kDa in size corresponding to S100 monomer by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full-length bovine S100 protein (mixture of aa homodimers and ab heterodimers).
• Target: S100A1
• Purity: This Protein A purified antibody is directed against bovine S100 protein. The product was purified from monospecific antiserum by Protein A chromatography. A BLAST analysis was used to suggest reactivity with this protein from bovine based on 100% homology with the immunogen sequence. A 98% homology is noted for S100 alpha chain from primate sources. Mouse, rat and dog show 94% homology with the bovine S100 alpha sequence. Expect cross reactivity with S100 from most mammalian sources. Cross reactivity with S100 from other specific sources has not been determined. Homologies for the S100 beta chain are similar.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 5.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-S100 Protein Antibody, S100 calcium-binding protein A1, S-100 protein subunit alpha, S-100 protein alpha chain

Quick Database Links

Gene Symbol

S100A1

UniProt

P02639

RefSeq (Protein):NP_001092512.1