S-trityl-L-Cysteine

SKU: orb1303907

Description

S-trityl-L-cysteine is a potent and selective cell-permeable inhibitor of the mitotic kinesin Eg5 (KIF11). It is widely used in cell biology and cancer research to induce mitotic arrest and monopolar spindles in both in vitro and in vivo experimental models.

Research Area

Cell Biology, Signal Transduction

Key Properties

• CAS Number: 2799-07-7
• MW: 363.47
• Purity: 97.02%
• Formula: C₂₂H₂₁NO₂S
• SMILES: C(SC[C@@H](C(O)=O)N)(C1=CC=CC=C1)(C2=CC=CC=C2)C3=CC=CC=C3
• Target: Kinesin,Apoptosis
• Solubility: DMSO:Slightly soluble;Methanol:1 mg/mL (2.75 mM)

Bioactivity

• Target IC50:
  EG5:500 nm
• In Vitro:
  In vitro, S-trityl-L-Cysteine targets the catalytic domain of Eg5 and inhibits Eg5 basal and microtubule-activated ATPase activity as well as mant-ADP release. S-trityl-L-Cysteine is a tight binding inhibitor (estimation of K(i,app) <150 nm at 300 mm NaCl and 600 nm at 25 mm KCl). S-trityl-L-Cysteine binds more tightly than monastrol because it has both an approximately 8-fold faster association rate and approximately 4-fold slower release rate (6.1/microM/s and 3.6/s for S-trityl-L-Cysteine versus 0.78 /microM/s and 15/s for monastrol). S-trityl-L-Cysteine inhibits Eg5-driven microtubule sliding velocity in a reversible fashion with an IC50 of 500 nm.

Storage & Handling

• Storage: keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, Eg5, NSC83265, NSC-83265, NSC 83265, Kinesin, kinesin Eg5, inhibit, cell cycle arrest, 328542, antitumor activities, Apoptosis, ATPase activity, S-Tritylcysteine, StritylLCysteine, S-trityl-L-Cysteine, STLC, S trityl L Cysteine

Compound Identifiers

PubChem CID

76044