Ruscogenin

SKU: orb1222229

Description

Ruscogenin is a steroid sapogenin that has been found in O. japonicus with diverse biological activities and prevents monocrotaline-induced increases in mean arterial pressure (MAP), expression of IL-1β and TNF in plasma and lung, and endothelial cell apoptosis in pulmonary arterioles in a rat model of pulmonary hypertension.

Key Properties

• CAS Number: 472-11-7
• MW: 430.62
• Purity: >98% (HPLC)
• Formula: C₂₇H₄₂O₄
• SMILES: CC1CCC2(C(C3C(O2)CC4C3(CCC5C4CC=C6C5(C(CC(C6)O)O)C)C)C)OC1
• Target: COX
• Solubility: In Vitro: DMSO : 25 mg/mL (58.06 mM)

Bioactivity

• In Vivo:
  Animal model: MCAO/R Mice. Dosage: 10 mg/kg. Administration: Oral Gavage (i. g.). Result: Showed smaller infarct size, ameliorating histopathological damage by decreasing the cell loss, and significant increase in CBF (cerebral blood flow) compared to the model group. Resulted inhibiting the expression of IL-1β and Caspase-1, NLRP3 and TXNIP compared to the model group.
• In Vitro:
  Cell Viability Assay Cell line: bEnd.3 cell Concentration: 0.01 μM, 0.1 μM, 1 μM, 10 μM, 20 μM, 40 μM Incubation time: 24 h. Result: Showed that the cell viability reduction in OGD/R-induced bEnd.3 cell was significantly recovered. Immunofluorescence Cell line: bEnd.3 cell Concentration: 0.1 μM, 1 μM, 10 μM Incubation time: 24 h. Result: Demonstrated increase in the TEER value and inhibition the sodium fluorescein permeability. Western blot analysis. Cell line: bEnd.3 cell Concentration: 0.01 μM, 0.1 μM, 1 μM, 10 μM, 20 μM, 40 μM Incubation time: 24 h. Result: Demonstrated downregulation of the expression of IL-1β and Caspase-1 proteins, and inhibition in the expressions of NLRP3 and TXNIP. Immunofluorescence Cell line: bEnd.3 cell Concentration: 0.01 μM, 0.1 μM, 1 μM, 10 μM, 20 μM, 40 μM Incubation time: 4 h. Result: Demonstrated reduction in the production of ROS.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Ruscogenin

Quick Database Links

Gene Symbol

COX