Rubrofusarin gentiobioside

SKU: orb1297215

Description

Rubrofusarin gentiobioside is a research compound shown to downregulate TGF-β1 and fibronectin expression while inhibiting NF-κB DNA binding activity. These in vitro findings indicate its potential utility in studying the molecular pathways underlying AGE-related diabetic nephropathy and vascular complications.

Research Area

Signal Transduction, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 24577-90-0
• MW: 596.53
• Purity: 99.93%
• Formula: C₂₇H₃₂O₁₅
• SMILES: COc1cc(O[C@@H]2O[C@H](CO[C@@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)[C@@H](O)[C@H](O)[C@H]2O)c2c(O)c3c(cc2c1)oc(C)cc3=O
• Target: TGF-beta/Smad,ERK,NF-κB
• Solubility: DMSO:22.5 mg/mL (37.72 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (3.35 mM)

Bioactivity

• In Vitro:
  To examine the pharmacological effects of a butanol-soluble extract of CS under conditions of diabetic nephropathy, we evaluated the expression of transforming growth factor-beta1 (TGF-beta1) and fibronectin, key mediators of diabetic nephropathy, in mouse glomerular mesangial cells cultured in the presence of S100b (a specific ligand for receptor of advanced glycation end products). CS inhibited S100b-induced TGF-beta1 and fibronectin expression in mouse mesangial cells by suppressing activation of Smad2/3, extracellular signal-regulated kinase (ERK)/mitogen-activated protein kinase (MAPK), and oxidative stress. Moreover, CS suppressed nuclear factor-kappa B (NF-kappaB) activation in S100b-stimulated mouse mesangial cells. To identify the active compounds of CS, three major compounds, Rubrofusarin-6-O-beta-D-gentiobioside (CS-A), toralactone-9-O-beta-d-gentiobioside (CS-B), and cassiaside (CS-C), were tested in cells. Of these compounds, CS-A significantly decreased the expression of TGF-beta1 and fibronectin and NF-kappaB DNA binding activity

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ERK, Inhibitor, inhibit, NF-κB, NFκB, NFkB, NF-kB, Rubrofusarin gentiobioside, Rubrofusarin-6-O-b-D-gentiobioside, Rubrofusarin-6-O-beta-D-gentiobioside, Rubrofusarin-6-O-β-D-gentiobioside, TGF-beta, TGFbeta/Smad, TGF-b/Smad, TGFb, TGFbeta, Smad, radical?scavenging, TGFβ, TGF-β/Smad

Compound Identifiers

PubChem CID

503733