RS 09 TFA

SKU: orb1981505

Description

RS 09 TFA is a synthetic TLR4 agonist and LPS-mimetic peptide that activates NF-κB signaling, driving pro-inflammatory cytokine production in macrophages like RAW264.7 cells. It is utilized in immunology research to study macrophage polarization, neuroinflammation, and the disruption of ciliary neuroprotective pathways in both cellular and animal models.

Research Area

Immunology & Inflammation, Signal Transduction

Key Properties

• MW: 805.8
• Purity: 99.78% (May vary between batches)
• Formula: C₃₃H₅₀F₃N₉O₁₁
• SMILES: O=C([C@@H]1CCCN1C([C@@H](N)C)=O)N2CCC[C@H]2C(N[C@@H](CC3=CNC=N3)C(N[C@@H](C)C(N[C@@H](CC(C)C)C(N[C@@H](CO)C(O)=O)=O)=O)=O)=O.FC(F)(C(O)=O)F
• Target: TLR,Immunology/Inflammation related,NF-κB
• Solubility: DMSO:≥ 80 mg/mL;H2O:≥ 40 mg/mL;10% DMSO+40% PEG300+5% Tween-80+45% Saline:3.3 mg/mL (4.1 mM)

Bioactivity

• In Vivo:
  RS 09 TFA serves as an immune adjuvant, combined with the prostate cancer-specific antigen X-15 (conjugated to the carrier protein KLH) for immunization in BALB/c mice. Experimental results demonstrate that this compound can significantly elevate the concentration of X-15-specific antibodies in the mice .
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• In Vitro:
  In HEK-BLUETM-4 cells, RS 09 TFA (1-10 μg/mL, 24 hours) can stimulate TLR-4 and activate NF-κB. In RAW264.7 cells, RS 09 TFA (5 μg/mL, 15 minutes-24 hours) binds to TLR-4 and induces NF-κB nuclear translocation as well as the secretion of inflammatory cytokines.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RS09 TFA, RS-09 TFA

Quick Database Links

Gene Symbol

TLR,Immunology/Inflammation related,NF-κB