Romidepsin

SKU: orb1226613

Description

Romidepsin (FK228, depsipeptide) is a potent HDAC1 and HDAC2 inhibitor with IC50 of 36 nM and 47 nM, respectively.(In Vitro):Romidepsin (0-72 hours; 0-80 nM) inhibits proliferation of HCC cells in dose-dependent manner.Romidepsin (0-48 hours; 0-60 nM) leads to a time- and dose-dependent induction of cell cycle arrest in the G2/M phase in HCC cells.Romidepsin (0-48 hours; 0-60 nM) promotesapoptosis in HCC cells, increases c-caspase-3, c-caspase-9, and c-PARP protein expression.(In Vivo):Romidepsin (intraperitoneal injection; 0.5 and 1 mg/kg; every 3 day; 21 days) inhibited the tumor growth, reveals a higher expression of p-cdc25C, ki67, c-caspase-3 and c-PARP, and a lower expression of Ki-67 in Romidepsin treated tumors .

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 128517-07-7
• MW: 540.7
• Purity: >98% (HPLC)
• Formula: C₂₄H₃₆N₄O₆S₂
• SMILES: O=C([C@H](C(C)C)NC(/C(N1)=C/C)=O)O[C@](CC(N[C@H](C(C)C)C(N2)=O)=O)([H])/C=C/CCSSC[C@]2([H])C1=O
• Target: HDAC
• Solubility: DMSO: 10 mg/mL (18.49 mM)

Bioactivity

• In Vivo:
  Animal model: Nude mice with Huh7 cells. Dosage: 0.5 and 1 mg/kg. Administration: Intraperitoneal injection; 0.5 and 1 mg/kg; every 3 day; 21 days. Result: Suppressed tumor growth in mouse xenograft models.
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• In Vitro:
  Cell Proliferation Assay Cell line: HCC cells. Concentration: 0 nM; 10 nM; 20 nM; 30 nM; 40 nM; 50 nM; 60 nM; 70 nM; 80 nM. Incubation time: 0 hours; 12 hours; 24 hours; 48 hours; 72 hours. Result: Inhibited HCC cells proliferation. Cell Cycle Analysis Cell line: HCC cells. Concentration: 0 nM; 15 nM; 30 nM; 60 nM. Incubation time: 12 hours; 24 hours; 48 hours. Result: Caused a G2/M arrest. Western blot analysis. Cell line: HCC cells. Concentration: 0 nM; 15 nM; 30 nM; 60 nM. Incubation time: 12 hours; 24 hours; 48 hours. Result: Increaesd c-caspase-3, c-caspase-9, and c-PARP expression in HCC cells.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FK228, Depsipeptide

Quick Database Links

Gene Symbol

HDAC