Rifampicin

SKU: orb1310391

Description

Rifampicin is a broad-spectrum antibiotic with potent activity against Mycobacterium tuberculosis, as well as various gram-positive and gram-negative bacteria. It is widely used in tuberculosis research and in vitro studies of bacterial RNA polymerase inhibition.

Research Area

Infectious Disease & Virology, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 13292-46-1
• MW: 822.94
• Purity: 99.28% (May vary between batches)
• Formula: C₄₃H₅₈N₄O₁₂
• SMILES: OC1=C2C3=C4O[C@@](C)(C3=O)O/C=C\[C@H](OC)[C@@H](C)[C@@H](OC(C)=O)[C@H](C)[C@H](O)[C@H](C)[C@@H](O)[C@@H](C)/C=C\C=C(/C)\C(=O)NC(C(O)=C2C(O)=C4C)=C1C=NN5CCN(C)CC5
• Target: Virus Protease,Antibacterial,Antibiotic,Influenza Virus
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:5 mg/mL (6.08 mM);DMSO:87.5 mg/mL (106.33 mM);Ethanol:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• Target IC50:
  HEK293 cells:0.3 μM|OATP1B1:1.2–1.5 μM|Osteoblast:130 μg/mL|HepG2 cells:145.33 μM|Huh7 cells:> 20 μM|Vero cells:> 10 μg/mL|HeLa cells:270 μg/mL|Macrophages:> 100 μM|MRC5 cells:> 100 μM|J774 cells.A1:> 64 μg/mL|MDCK cells-II:> 20 μM|PBMC cells:62.92 μg/mL|MG-63 cells:240 μg/mL|Hepatocyte cells:> 20 μM|THP-1 cells:0.0018 μg/mL
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• In Vivo:
  Rifampicin (200, 400 mg/kg) can induce fatty liver at high concentrations . Rifampicin (30 mg/kg, i.p.) treatment of S464P biofilms in vivo causes a slight decline in bioluminescence from these catheters compared with the parental signal, but it does not affect bioluminescence in mice infected with mutant H481Y .
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• In Vitro:
  METHODS: PC12 cells were treated with Rifampicin (25-100 µM) for 3-24 h. The expression levels of target proteins were detected by Western Blot. RESULTS: At 6 h of incubation, Rifampicin induced a significant increase in GRP78 protein expression in PC12 cells. Prolonged incubation with Rifampicin further enhanced the induction of GRP78 up to 24 h. PC12 cells showed a dose-dependent induction of GRP78 when incubated with Rifampicin at concentrations in the 25-100 µM range. METHODS: Human colon cancer cells LS180 were treated with Rifampicin (10 µM) for 24-144 h. Gene levels were measured by RT-PCR. RESULTS: The total expression of NR1I2 mRNA was reduced by 40% after 24 h of Rifampicin treatment, and the expression of PXR.3 mRNA was reduced by 45% after 24 h of Rifampicin treatment.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNA/RNA Synthesis, Antibiotic, Bacterial, 14-α Demethylase, Inhibitor, Influenza Virus, inhibit, Rifampicin, Rifampin, Rifamycin AMP, Rimactane, Orthopoxvirus

Compound Identifiers

PubChem CID

171360673