Ribavirin

SKU: orb1310388

Description

Ribavirin is a synthetic nucleoside analog with broad-spectrum antiviral activity, notably against hepatitis C and other RNA viruses. It is widely used in both in vitro virology studies and in vivo research models to investigate antiviral mechanisms and combination therapies.

Research Area

Immunology & Inflammation

Key Properties

• CAS Number: 36791-04-5
• MW: 244.21
• Purity: >98%
• Formula: C₈H₁₂N₄O₅
• SMILES: NC(=O)C1=NN(C=N1)[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O
• Target: Dehydrogenase (GAPDH, GPDH, IMPDH, PHGDH, LDH, DHODH, ALDH, GDH, IDH, UGDH, DPD, DHPDH, KGDH, PDH, and MDH)
• Solubility: Soluble in DMSO (up to 25 mg/ml) or in Water (up to 25 mg/ml).

Bioactivity

• Target IC50:
  K562 cells:78.30 µM|A549 cells:3 μM (EC50)|SUP-B15 cells:65.18 µmol/ L|Macrophages (anti-inflammatory):19 μM|Macrophages (anti-inflammatory):7 μM (EC50)
• In Vivo:
  METHODS: To investigate the antiviral effect against CCHF virus, Ribavirin (100 mg/kg) was administered intraperitoneally to CCHF virus-infected IFNAR-/- mice once daily until death or day 8. RESULTS: CCHF virus-infected IFNAR-/- mice died 2-6 days post-infection, with elevated blood and organ transaminase levels and high viral titers.Ribavirin did not increase the survival rate of the IFNAR-/- mice but prolonged the time to death and decreased the transaminase levels and viral titers.
• Cell Research:
  The effect of Ribavirin on microglial cell viability is evaluated by the sulforhodamine B (SRB) chemosensitivity assay. Briefly, LPS-stimulated microglial cells are incubated for 48 h in the presence or absence of Ribavirin. Afterward, the cells are fixed in 10% (w/v) trichloroacetic acid for 1 h at 4°C, rinsed in tap water and stained with 0.4% (w/v) SRB in 1% acetic acid (100 μL/well) for 30 min at room temperature (RT). The cells are then rinsed three times in 1% acetic acid to remove the unbound stain. The protein bound stain is extracted with 200 μL 10 mM Tris base (pH 10.5) per well. The optical density is read at 540 nm, with correction at 670 nm. The results are presented as percentage of the control (non-stimulated/untreated microglial cells), that is arbitrarily set to 100%.

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNASynthesis, DNA Synthesis, Antibiotic, ADK, NSC163039, NSC-163039, Orthopoxvirus, IMPDH1, NSC 163039, inhibit, Inhibitor, ICN 1229, ICN1229, ICN-1229, Hepatitis C virus, HCVProtease, HCV, HCV Protease, Tribavirin, RNA polymerase, RNA Synthesis, RNASynthesis, RTCA, RSV, Respiratory syncytial virus, Ribavirin

Compound Identifiers

PubChem CID

37542