Ribavirin

SKU: orb1310388

Description

Ribavirin

Research Area

Cell Biology, Infectious Disease & Virology, Metabolism Research, Molecular Biology, Neuroscience, Protein Biochemistry

Key Properties

• CAS Number: 36791-04-5
• MW: 244.20
• Purity: 99.83%
• Formula: C₈H₁₂N₄O₅
• SMILES: NC(=O)C1=NN(C=N1)[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O
• Target: Virus Protease,HCV Protease,Dehydrogenase,Antibiotic,AChR,DNA/RNA Synthesis,RSV
• Solubility: H2O:24.4 mg/mL (99.92 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:5 mg/mL (20.48 mM);DMSO:257.5 mg/mL (1054.46 mM)

Bioactivity

• Target IC50:
  K562 cells:78.30 µM A549 cells:3 μM (EC50)|SUP-B15 cells:65.18 µmol/ L|Macrophages (anti-inflammatory):19 μM|Macrophages (anti-inflammatory):7 μM (EC50)
• In Vivo:
  METHODS: To investigat the antiviral effect against CCHF virus, Ribavirin (100 mg/kg) was administered intraperitoneally to CCHF virus-infected IFNAR-/- mice once daily until death or day 8. Results: CCHF virus-infected IFNAR-/- mice died 2-6 dayss post-infection, with elevated blood and organ transaminase levels and high viral titers.Ribavirin did not increas the survival rate o the IFNAR-/- mice but prolonge the time to death and decrease the transaminase levels and viral titers.
• Cell Research:
  The effect of Ribavirin on microglial cell viability is evaluated b the sulforhodamine B (SRB) chemosensitivity assay. Briefly, LPS-stimulated microglial cells are incubated for 48 h in the presence or absence of Ribavirin. Afterward the cells are fixed in 10% (w/v) trichloroacetic acid for 1 h at 4°C, rinsed in tap water and stained with 0.4% (w/v) SRB in 1% acetic acid (100 μL well) for 30 min at room temperature (RT) the cells are then rinsed three times in 1% acetic acid to remov the unbound stain the protein bound stain is extracted with 200 μL 10 mM Tris base (pH 10.5) per well the optical density is read at 540 nM, with correction at 670 nM the results are presented as percentage o the control (non-stimulated/untreated microglial cells), that is arbitrarily set to 100%.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

DNASynthesis, DNA Synthesis, Antibiotic, ADK, NSC163039, NSC-163039, Orthopoxvirus, IMPDH1, NSC 163039, inhibit, Inhibitor, ICN 1229, ICN1229, ICN-1229, Hepatitis C virus, HCVProtease, HCV, HCV Protease, Tribavirin, RNA polymerase, RNA Synthesis, RNASynthesis, RTCA, RSV, Respiratory syncytial virus, Ribavirin

Compound Identifiers

PubChem CID

37542