Rhodamine-12-dUTP

• Catalog Number: orb1733875
• Category: Tools
• Description: Rhodamine-12-dUTP is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substi-tute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Rhodamine-12-dUTP/dTTP ratio for Nick Trans-lation: 30-50% Rhodamine-12-dUTP/70-50% dTTP.
• Form/Appearance: solution in water; Color: orange; pH: 7.5 ± 0.5
• Concentration: 1.0 mM-1.1 mM
• Purity: ≥ 95% (HPLC)
• MW: Theoretical MW: 992.71 g/mol (free acid); Detected MW: 992.18 g/mol (free acid)
• Application notes: Incorporation into DNA/cDNA by Nick Translation with DNAse I/DNA Polymerase Iin-house data. ; Spectroscopic Propertie: λexc 505 nm, λem 530 nm, ε 85.0 L mmol-1 cm-1 (Tris-HCl pH 7.5), CF 280 nm 0.27, CF 260 nm 0.39.
• Formula: C₃₉H₄₃N₆O₁₉P₃
• SMILES: [nH]1c(=O)c(cn(c1=O)[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)C1)/C=C/CNC(=O)CCCCCNC(=O)c1ccc(c(c1)c1c2ccc(cc2oc2-c1ccc(=[NH2+])c2)N)C(=O)[O-]
• Storage: store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
• Note: For research use only
• Expiration Date: 12 months from date of receipt.