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Catalog Number | orb1733875 |
---|---|
Category | Tools |
Description | Rhodamine-12-dUTP is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substi-tute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Rhodamine-12-dUTP/dTTP ratio for Nick Trans-lation: 30-50% Rhodamine-12-dUTP/70-50% dTTP. |
Concentration | 1.0 mM-1.1 mM |
Form/Appearance | solution in water; Color: orange; pH: 7.5 ± 0.5 |
Purity | ≥ 95% (HPLC) |
MW | Theoretical MW: 992.71 g/mol (free acid); Detected MW: 992.18 g/mol (free acid) |
SMILES | [nH]1c(=O)c(cn(c1=O)[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)C1)/C=C/CNC(=O)CCCCCNC(=O)c1ccc(c(c1)c1c2ccc(cc2oc2c1ccc(=[NH2+])c2)N)C(=O)[O-] |
Formula | C39H43N6O19P3 |
Storage | store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible |
Note | For research use only |
Application notes | Incorporation into DNA/cDNA by Nick Translation with DNAse I/DNA Polymerase Iin-house data.
Spectroscopic Propertie: λexc 505 nm, λem 530 nm, ε 85.0 L mmol-1 cm-1 (Tris-HCl pH 7.5), CF 280 nm 0.27, CF 260 nm 0.39. |
Expiration Date | 12 months from date of receipt. |
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