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Rhesus macaque CD47 Recombinant Protein

SKU: orb1231158
FeaturedFeatured Product

Description

Rhesus macaque CD47 Recombinant Protein

Research Area

Cancer Biology, Immunology & Inflammation, Neuroscience

Images & Validation

Tested ApplicationsELISA, WB
Application Notes
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 15.8 kDa. The protein migrates as 30-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

SourceHEK293 cells
TargetCD47
TagHis Tag
Molecular Weight15.8 kDa
Protein SequenceGln 19 - Glu 141
Purity>95% as determined by SDS-PAGE.

Storage & Handling

StorageLyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles.
Form/AppearanceLyophilized
Buffer/PreservativesPBS, pH 7.4
DisclaimerFor research use only

Alternative Names

CD47,MER6,IAP,OA3

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    SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

    Predicted: 15.7 KDa. Observed: 33-55 KDa, reducing conditions

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  • Rhesus macaque CD47 [orb2994745]

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    SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

    Predicted: 40.8 KDa. Observed: 60-90 KDa, reducing conditions

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Rhesus macaque CD47 Recombinant Protein

Cynomolgus/Rhesus macaque CD47, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Rhesus macaque CD47 Recombinant Protein

Immobilized Human SIRP alpha, Fc Tag (HPLC-verified) at 5 ug/ml (100 uL/well) can bind Cynomolgus/Rhesus macaque CD47, His Tag with a linear range of 4-63 ng/mL.

Rhesus macaque CD47 Recombinant Protein

Immobilized Anti-CD47, Human IgG4 at 2 ug/ml (100 uL/well) can bind Cynomolgus/Rhesus macaque CD47, His Tag with a linear range of 0.2-5 ng/mL.

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Documents Download

Datasheet
Product Information
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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Rhesus macaque CD47 Recombinant Protein (orb1231158)

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Available Sizes

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0.05 mg
$ 1,060.00
DispatchUsually dispatched within 5-10 working days
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