RG14620

SKU: orb1304071

Description

RG14620 (Tyrphostin RG14620) is a small molecule inhibitor targeting the epidermal growth factor receptor (EGFR). It is used in biochemical and cellular research to study EGFR signaling pathways in both in vitro assays and in vivo experimental models.

Research Area

Cardiovascular Research, Signal Transduction

Key Properties

• CAS Number: 136831-49-7
• MW: 275.13
• Purity: 99.87% (May vary between batches)
• Formula: C₁₄H₈Cl₂N₂
• SMILES: Clc1cc(Cl)cc(C=C(C#N)c2cccnc2)c1
• Target: HER,EGFR
• Solubility: DMSO:18.33 mg/mL (66.62 mM);10% DMSO+90% Corn Oil:1.5 mg/mL (5.45 mM)

Bioactivity

• Target IC50:
  EGFR:3 μM.
• In Vivo:
  RG-14620, at a dose of 200 μg/mouse/day inhibits MH-85 tumor growth in nude mice. Mice show less cachexia and hypercalcemia, eat more food, and are more active than untreated MH-85 tumor-bearing animals.
• In Vitro:
  RG-14620 inhibits colony formation and DNA synthesis by HER 14 cells, which are stimulated by 50 ng/mL EGF, in a dose-dependent manner. The IC50s for RG-14620 are 3 μM for HER 14 colony formation and 1 pM for HER 14 DNA synthesis. RG-14620 also suppresses colony formation and DNA synthesis by EGF-stimulated MH-85 cells in a dose-dependent manner. The IC50s for RG-14620 on MH-85 cells are 4 μM for colony formation and 1.25 μM for DNA synthesis.
• Cell Research:
  RG14620 is dissolved in 100% DMSO and diluted with the culture medium before addition to the cells. MH-85 and HER 14 cells are cultured in the medium in the presence or absence of increasing concentrations of RG-13022 or RG-14620 for 10 days. At the end of culture, the cells are fixed with 4% (v/v) formaldehyde in calcium-magnesium-free phosphate-buffered saline for 15 min at room temperature and stained with hematoxylin. Numbers of colonies including more than 20 cells in each well are counted under the microscope.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ErbB-1, Epidermal growth factor receptor, EGFR, Inhibitor, HER1, HER14 colony formation, HER14 DNA synthesis, inhibit, RG14620, RG-14620, RG 14620, Tyrphostin RG 14620, Tyrphostin RG14620, Tyrphostin RG-14620

Compound Identifiers

PubChem CID

5061