Retinol

SKU: orb1309490

Description

Retinol

Research Area

Metabolism Research, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 68-26-8
• MW: 286.45
• Purity: 95.29% (May vary between batches)
• Formula: C₂₀H₃₀O
• SMILES: C\C(=C/CO)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C
• Target: Others,Endogenous Metabolite
• Solubility: Ethanol:50 mg/mL (174.55 mM);DMSO:245 mg/mL (855.3 mM)

Bioactivity

• Target IC50:
  RARα:14 nM|RARγ:14 nM|RARβ:14 nM|RAW 264.7 cells:140 μM
• In Vivo:
  METHODS: To study placental pharmacokinetics and teratogenicity, Retinol (10-100 mg/kg) was administered by gavage to pregnant mice on day 11 of gestation. RESULTS: No teratogenic effects were observed after administration of 10 mg/kg Retinol. Pharmacokinetic studies showed moderate increases in retinyl esters, retinol, and all-trans-retinoic acid in plasma, embryonic tissues, placenta, yolk sac membranes, and extra-embryonic fluids. 100 mg/kg Retinol administration resulted in a high incidence of severe fetal malformations. There was a significant increase in retinyl esters and retinol in all compartments, including the embryo, and the polar metabolites all-trans-retinoic acid and all-trans-4-oxoretinoic acid were produced in large amounts.
• In Vitro:
  METHODS: The human SH-SY5Y neuronal cell line was treated with Retinol (1-20 µM) for 24 h. Cell viability was measured by MTT assay and SRB assay. RESULTS: Retinol at 7, 10 and 20 µM significantly reduced cell viability. concentrations below 5 µM did not affect MTT reduction or SRB doping in SH-SY5Y cells, but at 7 µM these parameters decreased, indicating a reduction in the number of viable cells compared to the control group. METHODS: Human hepatocellular carcinoma cells, HepG2, were treated with Retinol (1 µM) for 24 h. Retinol distribution was examined using Flow cytometry. RESULTS: After 1 day of treatment with 1 µM Retinol, the percentage of Retinol-positive HepG2 cells in the total cell population increased from 17.6% to 69.8%. Fluorescence analysis showed that Retinol was located in the cytoplasm of HepG2 cells.

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

all-trans-Retinol, Alphalin, Retinol, Retinol-binding protein, Vitamin A1

Compound Identifiers

PubChem CID

445354