RecombinantTNF-α,RhesusMacaque

SKU: orb1494765

Description

Tumor Necrosis Factor-Alpha (TNF-α) plays a major role in regulating growth, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune disease. TNF alpha-1a is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells. In addition to inducing hemorrhagic necrosis of tumors, studies indicate TNF is involved in certain types of tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, Crohn's disease, rheumatoid arthritis and graft-versus-host disease.Recombinant Rhesus Macaque TNF-α produced in E.coli is a single, non-glycosylated polypeptide chain containing 157 amino acids. A fully biologically active molecule, Recombinant Rhesus Macaque TNF-α has a molecular mass of 17.4 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.

Images & Validation

• Application Notes:
  Reconstituted in ddH2O at 100 μg/ml.

Key Properties

• Source: Escherichia coli.
• Biological Activity: ED50 1.6 × 10ˆ7 units/mg.
• Molecular Weight: 17.4 kDa, observed by reducing SDS-PAGE.
• Protein Sequence: VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELTDNQLVV PSEGLYLIYS QVLFKGQGCP SNHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINLPD YLDFAESGQV YFGIIAL
• Purification: > 95% as analyzed by SDS-PAGE& HPLC
• Purity: > 95% as analyzed by SDS-PAGE& HPLC
• Endotoxins: < 0.2 EU/μg, determined by LAL method.

Storage & Handling

• Storage: Lyophilized recombinant Rhesus Macaque TNF-α, remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Rhesus Macaque TNF-α should be stable up to 1 week at 4°C or up to 3 months at -20°C.
• Form/Appearance: Lyophilized after extensive dialysis against PBS
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS
• Disclaimer: For research use only

Alternative Names

TNF-alpha, TNF-α; Tumornecrosis factor ligand super family member 2, TNF-a; TNFa, Cachectin, DIF, TNFA, TNFSF 2