RecombinantTNF-α,His,Human

SKU: orb1494768

Description

Tumor Necrosis Factor-Alpha (TNF-alpha) plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases. Besides inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, and rheumatoid arthritis as well as graft-versus-host disease. TNF alpha-1a is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.Recombinant human Tumor Necrosis Factor-Alpha (rhTNF-alpha) produced in E. coli is a non-glycosylated polypeptide chain of 164 amino acids. A fully biologically active molecule, rhTNF-alpha has a molecular mass of 18.3kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.

Images & Validation

• Application Notes:
  Reconstituted in ddH2O at 100 μg/ml.

Key Properties

• Source: Escherichia coli.
• Biological Activity: ED507.69 x 10ˆ6units/mg.
• Molecular Weight: 18.3kDa, observed by reducing SDS-PAGE.
• Protein Sequence: MHHHHHHVR SSSRTPSDKP VAHVVANPQA EGQLQWLNRR ANALLANGVE LRDNQLVVPS EGLYLIYSQV LFKGQGCPST HVLLTHTISR IAVSYQTKVN LLSAIKSPCQ RETPEGAEAK PWYEPIYLGG VFQLEKGDRL SAEINRPDYL DFAESGQVYF GIIAL
• Purification: > 95% by SDS-PAGE analysis.
• Purity: > 95% by SDS-PAGE analysis.
• Endotoxins: < 1.0 EU/μg, determined by LAL method.

Storage & Handling

• Storage: Lyophilized recombinant human Tumor Necrosis Factor-Alpha (rhTNF-alpha) remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rhTNF-alpha should be stable up to 4 weeks at 4°C or up to 6 months at -20°C.
• Form/Appearance: Lyophilized after extensive dialysis against PBS.
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

TNF-alpha, TNF-α; Tumornecrosis factor ligand super family member 2, TNF-a; TNFa, Cachectin, DIF, TNFA, TNFSF 2