RecombinantsIL-6Rα,His,Human

SKU: orb1494598

Description

Interleukin-6 Receptor (IL-6R) is a single trans-membrane protein that is the receptor for Interleukin-6 (IL-6). IL-6R forms a hexameric complex upon binding 2 molecules of IL-6 and two molecules of glycoprotein 130 (gp130) which activates intracellular JAK/STAT pathways. Although the normal form of IL-6R is the membrane-bound 80 kDa subunit, a soluble form of IL-6R (sIL-6R) can be generated physiologically by limited proteolysis or alternative splicing. sIL-6R binds to both IL-6 and gp130 generating intracellular signaling. In the immune system, sIL-6R is produced by both naïve and memory CD4 T-cells and strongly augments IL-6 ligand’s induction of Th-17 cells.Recombinant human Soluble IL-6 Receptor Alpha (rhsIL-6R alpha) with an N-terminal His tag produced in Sf9 insect cells is a single glycosylated polypeptide chain containing 350 amino acids. A fully biologically active molecule, rhsIL-6R alpha has a molecular mass of 50kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Images & Validation

• Application Notes:
  Reconstituted in ddH2O at 100 μg/mL.

Key Properties

• Source: SF9 insect cells
• Biological Activity: ED50 2×10ˆ4 units/mg.
• Molecular Weight: 50 kDa, observed by reducing SDS-PAGE.
• Protein Sequence: HHHHHHD DDDKLAPRRC PAQEVARGVL TSLPGDSVTL TCPGVEPEDN ATVHWVLRKP AAGSHPSRWA GMGRRLLLRS VQLHDSGNYS CYRAGRPAGT VHLLVDVPPE EPQLSCFRKS PLSNVVCEWG PRSTPSLTTK AVLLVRKFQN SPAEDFQEPC QYSQESQKFS CQLAVPEGDS SFYIVSMCVA SSVGSKFSKT QTFQGCGILQ PDPPANITVT AVARNPRWLS VTWQDPHSWN SSFYRLRFEL RYRAERSKTF TTWMVKDLQH HCVIHDAWSG LRHVVQLRAQ EEFGQGEWSE WSPEAMGTPW TESRSPPAEN EVSTPMQALT TNKDDDNILF RDSANATSLP VQD
• Purification: > 95% by SDS-PAGE and HPLC analyses.
• Purity: > 95% by SDS-PAGE and HPLC analyses.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.

Storage & Handling

• Storage: Lyophilized recombinant human Soluble IL-6 Receptor Alpha (rhsIL-6R alpha) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhsIL-6R alpha remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
• Form/Appearance: Lyophilized after extensive dialysis against PBS.
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

Soluble IL-6 Receptor alpha; gp80