RecombinantOSM(227aa),Human

SKU: orb1494783

Description

Oncostatin M (OSM) is a multifunctional cytokine, and belongs to Interleukin-6 (IL-6) subfamily, including IL-11, leukemia inhibitory factor (LIF), ciliary neurotropic factor, cardiotrophin-1, and novel neurotropin-1. In vivo, OSM is secreted from activated T cells, monocytes, neutrophils, and endothelial cells. OSM is related to LIF, and share a receptor with LIF in human. Human OSM can bind to gp130 and recruit OSM Receptor β or LIF Receptor β to form a ternary complex. OSM stimulates the growth of different types of cells, including megakaryocytes, fibroblasts, vascular endothelial cells, and T cells. On the other hand, OSM inhibits the proliferation of several cancer cell lines, such as solid tissue tumor cells, lung cancer cells, melanoma cells, and breast cancer cells.Recombinant human Oncostatin M (rhOSM) produced in E.coli is a single non-glycosylated polypeptide chain containing 228 amino acids. A fully biologically active molecule, rhOSM has a molecular mass of 25.9 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Images & Validation

• Application Notes:
  Reconstituted in ddH2O at 100 μg/mL.

Key Properties

• Source: Escherichia coli.
• Biological Activity: ED50 1× 10ˆ5 units/mg.
• Molecular Weight: 25.9 kDa, observed by reducing SDS-PAGE.
• Protein Sequence: MAAIGSCSK EYRVLLGQLQ KQTDLMQDTS RLLDPYIRIQ GLDVPKLREH CRERPGAFPS EETLRGLGRR GFLQTLNATL GCVLHRLADL EQRLPKAQDL ERSGLNIEDL EKLQMARPNI LGLRNNIYCM AQLLDNSDTA EPTKAGRGAS QPPTPTPASD AFQRKLEGCR FLHGYHRFMH SVGRVFSKWG ESPNRSRRHS PHQALRKGVR RTRPSRKGKR LMTRGQLPR
• Purification: > 95% by SDS-PAGE analysis.
• Purity: > 95% by SDS-PAGE analysis.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.

Storage & Handling

• Storage: Lyophilized recombinant human Oncostatin M (rhOSM) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhOSM should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
• Form/Appearance: Lyophilized after extensive dialysis against PBS.
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

Oncostatin M