RecombinantMIP-3α/CCL20,Human

SKU: orb1494790

Description

Chemokine (C-C motif) ligand 20 (CCL20) or liver activation regulated chemokine (LARC) or Macrophage Inflammatory Protein-3 (MIP3A) is a small cytokine belonging to the CC chemokine family. It is strongly chemotactic for lymphocytes and weakly attracts neutrophils, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus. It can signal through the CCR6 receptor. MIP-3 alpha is chemotactic towards lymphocytes and dendritic cells. Additionally, it promotes the adhesion of memory CD4+ T cells and inhibits colony formation of bone marrow myeloid immature progenitors.Recombinant Human MIP-3α/CCL20 produced in E.coliis a single non-glycosylated polypeptide chain containing 70 amino acids. A fully biologically active molecule, rhMIP-3α/CCL20has a molecular mass of 8.0 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.

Images & Validation

• Application Notes:
  Reconstituted in ddH2O or PBS at 100 μg/ml.

Key Properties

• Source: Escherichia coli.
• Biological Activity: The EC50 value of humanMIP-3 alpha/CCL20 on Caˆ2+ mobilization assay in CHO-K1/G15/hCCR6 cells (human Ga15 and human CCR6 stably expressed in CHO-K1 cells) is less than 200 ng/ml.
• Molecular Weight: 8.0 kDa, observed by reducing SDS-PAGE.
• Protein Sequence: ASN FDCCLGYTDR ILHPKFIVGF TRQLANEGCD INAIIFHTKK KLSVCANPKQ TWVKYIVRLL SKKVKNM
• Purification: > 95% as analyzed by SDS-PAGE.
• Purity: > 95% as analyzed by SDS-PAGE.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.

Storage & Handling

• Storage: Lyophilized recombinant Human MIP- 3α/CCL20 remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, recombinant human MIP-3α/CCL20 should be stable up to 1 week at 4°C or up to 2 months at -20°C.
• Form/Appearance: Lyophilized after extensive dialysis against 53mM Na2HPO4, 147 mM NaH2PO4, 300 mM NaCl, pH 6.5.
• Buffer/Preservatives: Lyophilized after extensive dialysis against 53mM Na2HPO4, 147 mM NaH2PO4, 300 mM NaCl, pH 6.5.
• Disclaimer: For research use only

Alternative Names

MIP3α, MIP-3 alpha, CCL20