RecombinantMIF,Mouse

SKU: orb1494794

Description

Macrophage Migration Inhibitory Factor (MIF) is a pleiotropic cytokine, existing as a homotrimer in vivo. MIF was originally identified as a T cell derived factor responsible for the inhibition of macrophage migration. However, recently MIF has received much more attention because of its possible roles in angiogenesis and cancer development. MIF is over-expressed in various cancers, including pancreatic, breast, colon, brain, prostate, skin, and lung. The intratumoral expression MIF is strongly correlated with angiogenic growth factor expression, such as the expression of Interleukin 8 (IL-8) and Vascular Endothelial Growth Factor (VEGF), and with risk of recurrence after resection. Recombinant mouse Macrophage Migration Inhibitory Factor (rmMIF) produced in E.coli is a single non-glycosylated polypeptide chain containing 115 amino acids. rmMIF has a molecular mass of 12.5 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Images & Validation

• Application Notes:
  Reconstituted in ddH2O at 100 μg/mL.

Key Properties

• Source: Escherichia coli.
• Biological Activity: Bioassay data are not available.
• Molecular Weight: 12.5 kDa, observed by reducing SDS-PAGE.
• Protein Sequence: MPMFIVNTN VPRASVPEGF LSELTQQLAQ ATGKPAQYIA VHVVPDQLMT FSGTNDPCAL CSLHSIGKIG GAQNRNYSKL LCGLLSDRLH ISPDRVYINY YDMNAANVGW NGSTFA
• Purification: > 95% as analyzed by SDS-PAGE and HPLC.
• Purity: > 95% as analyzed by SDS-PAGE and HPLC.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.

Storage & Handling

• Storage: Lyophilized recombinant mouse Macrophage Migration Inhibitory Factor (rmMIF) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmMIF remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
• Form/Appearance: Lyophilized after extensive dialysis against PBS.
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

Macrophage Migration, Inhibitory Factor, GLIF, MMIF, GIF, Glycosylation-inhibiting factor