RecombinantIL-1β,Porcine

• Catalog Number: orb1494827
• Category: Proteins
• Description: Interleukin-1 beta (rpIL-1β) is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. IL-1α and IL-1β are structurally related polypeptides that share approximately 27% amino acid (aa) identity in porcine. While IL-1α and IL-1β are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1β stimulates thymocyte proliferation by inducing il-2 release, b-cell maturation and proliferation, and fibroblast growth factor activity.Recombinant Porcine IL-1β produced in E.coli is a single non-glycosylated polypeptide chain containing 154 amino acids. A fully biologically active molecule, rpIL-1β has a molecular mass of 17.7 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
• Form/Appearance: Lyophilized after extensive dialysis against PBS.
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS.
• Purity: > 95% as analyzed by SDS-PAGE.
• Purification: > 95% as analyzed by SDS-PAGE.
• Protein Sequence: MANVQSMEC KLQDKDHKSL VLAGPHMLKA LHLLTGDLKR EVVFCMSFVQ GDDSNNKIPV TLGIKGKNLY LSCVMKDNTP TLQLEDIDPK RYPKRDMEKR FVFYKTEIKN RVEFESALYP NWYISTSQAE QKPVFLGNSK GRQDITDFTM EVLSP
• MW: 17.7 kDa, observed by reducing SDS-PAGE.
• Application notes: Reconstituted in ddH2O or PBS at 100 μg/ml.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.
• Source: Escherichia coli.
• Biological Activity: ED50 < 1ng/ml, measured by the dose-dependent stimulation of mouse D10S cells, corresponding to a specific activity of 1.0× 10ˆ6 IU/mg.
• Storage: Lyophilized recombinant Porcine IL-1β, remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Porcine IL-1β should be stable up to 1 week at 4°C or up to 3 months at -20°C.
• Alternative names: Interleukin-1β; IL-1β; Catabolin, Lymphocyte-activ
• Note: For research use only