RecombinantIFN-α2a,Human

SKU: orb1494845

Description

Interferon-Alpha 2a (IFN-Alpha 2a), Human produced by leukocytes is a member of Interferon family. IFN-alpha is mainly involved in innate immune response against a broad range of viral infections. IFN-alpha 2 has three acid stable forms (a,b,c) signaling through IFNAR2. IFN-alpha 2a shares 99.4% , 98.8% aa sequence identity with IFN-alpha 2b and 2c respectively. IFN-alpha contains four highly conserved cysteine residues which form two disulfide bonds, one of which is necessary for biological activity.Recombinant Interferon-Alpha 2a (IFN-Alpha 2a), Human produced in E.coli is a single non-glycosylated polypeptide chain containing 165 amino acids. A fully biologically active molecule, rhIFN-Alpha has a molecular mass of 19.2kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Images & Validation

• Application Notes:
  Reconstituted in ddH2O at 100 μg/ml.

Key Properties

• Source: Escherichia coli.
• Biological Activity: ED50 1.0× 10ˆ7 units/mg.
• Molecular Weight: 19.2kDa, observed by reducing SDS-PAGE.
• Protein Sequence: CDL PQTHSLGSRR TLMLLAQMRK ISLFSCLKDR HDFGFPQEEF GNQFQKAETI PVLHEMIQQI FNLFSTKDSS AAWDETLLDK FYTELYQQLN DLEACVIQGV GVTETPLMKE DSILAVRKYF QRITLYLKEK KYSPCAWEVV RAEIMRSFSL STNLQESLRS KE
• Purification: > 95% by SDS-PAGE and HPLC analyses.
• Purity: > 95% by SDS-PAGE and HPLC analyses.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.

Storage & Handling

• Storage: Lyophilized recombinant human Interferon-Alpha 2a (rhIFN-Alpha 2a) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIFN-Alpha 2a should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
• Form/Appearance: Lyophilized after extensive dialysis against PBS.
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

Leukocyte interferon, B cell interferon, Type I interferon, IFNA2, IFN-a 2a.