RecombinantGM-CSF(Sargramostim),Human(P.pastoris-expressed)

• Catalog Number: orb1494614
• Category: Proteins
• Description: Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF), a hematopoietic growth factor, is mainly involved in granulopoiesis and monocytopoiesis. It is produced by T-cells and macrophages in response to antigens, and by endothelial cells and fibroblasts following induction of variouscytokines [1]. A monomeric protein of 127 amino acidswith six glycosylation sites and two intra disulfide bonds[2,3], glycosylated and non-glycosylated hGM-CSFs show similar biological activities[4]. Other than its connection to the growth and development of granulocytes and macrophages, it is also indispensable for the proliferation of erythroid and megakaryocytic cells[5].Recombinant Human Granulocyte Macrophage Colony Stimulating Factor (rhGM-CSF) produced in P. pastoris a glycosylated polypeptide. A fully biologically active molecule, rhGM-CSF has a molecular mass of 26-32kDa analyzed by SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.
• Form/Appearance: Lyophilized after extensive dialysis against 10 mM PB, pH 7.0.
• Buffer/Preservatives: Lyophilized after extensive dialysis against 10 mM PB, pH 7.0.
• Purity: > 95 % as analyzed by SDS-PAGE.
• Purification: > 95 % as analyzed by SDS-PAGE.
• Protein Sequence: MAPARS PSPSTQPWEH VNAIQEALRL LNLSRDTAAE MNETVEVISE MFDLQEPTCL QTRLELYKQG LRGSLTKLKG PLTMMASHYK QHCPPTPETS CATQIITFES FKENLKDFLL VIPFDCWEPV QE
• MW: 26-32kDa, observed by SDS-PAGE.
• Application notes: Reconstituted in ddH2O at 100 μg/ml.
• Endotoxins: < 1.0EU/μg, determined by LAL method.
• Source: P. pastoris
• Biological Activity: ED50 5x 10ˆ6 units/mg.
• Storage: Lyophilized recombinant human Granulocyte Macrophage Colony Stimulating Factor (rhGM-CSF) remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rhGM-CSF should be stable up to 4 weeks at 4°C or up to 6 months at -20°C.
• Alternative names: Granulocyte Macrophage Colony Stimulating Factor,
• Note: For research use only
• Expiration Date: 6 months from date of receipt.