RecombinantG-CSF,Rat

• Catalog Number: orb1494859
• Category: Proteins
• Description: Granulocyte Colony-Stimulating Factor (G-CSF) is a hematopoietic cytokine belonging to the four-helix bundle cytokine superfamily. G-CSF is produced by monocytes, macrophages, fibroblasts, and endothelial cells. Its expression is highly regulated and induced by a variety of agents, including Tumor Necrosis Factor (TNF), Interleukin-1 (IL-1), Interferon γ (IFN-γ), and Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF). G-CSF binds to the CSF-specific high affinity receptors expressed on neutrophilic granulocyte lineage. In vivo G-CSF regulates the production of neutrophilic granulocytes, a critical part of host defense systems, and helps the maturation of leukemic cell lines. G-CSF is widely employed clinically because of its fairly innocuous safety profile.Recombinant rat Granulocyte Colony-Stimulating Factor (rrG-CSF) produced in E.coli is a single non-glycosylated polypeptide chain containing of 194 amino acids. A fully biologically active molecule, rrG-CSF has a molecular mass of 21.4 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
• Form/Appearance: Lyophilized after extensive dialysis against 20mM Citric Acid
• Buffer/Preservatives: Lyophilized after extensive dialysis against 20mM Citric Acid
• Purity: > 95% by SDS-PAGE analysis.
• Purification: > 95% by SDS-PAGE analysis.
• Protein Sequence: MIPLLTVSS LPPSLPLPRS FLLKSLEQVR KIQARNTELL EQLCATYKLC HPEELVLFGH SLGIPKASLS SCSSQALQQT KCLSQLHSGL FLYQGLLQAL AGISSELAPT LDMLHLDVDN FATTIWQQME SLGVAPTVQP TQSTMPIFTS AFQRRAGGVL VTSYLQSFLE TAHHALHHLP RPAQKHFPES LFISI
• MW: 21.4 kDa, observed by non-reducing SDS-PAGE.
• Application notes: Reconstituted in ddH2O at 100 μg/mL.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.
• Source: Escherichia coli.
• Biological Activity: ED50 1 × 10ˆ7 units/mg.
• Storage: Lyophilized recombinant rat Granulocyte Colony-Stimulating Factor (rrG-CSF) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rrG-CSF remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
• Alternative names: Granulocyte Colony-Stimulating Factor, CSF-3, CSF3
• Note: For research use only
• Expiration Date: 6 months from date of receipt.