RecombinantENA-78/CXCL5(5-78aa),Human

SKU: orb1494891

Description

Epithelial cell­derived neutrophil­activating peptide (ENA­78) also known as C-X-C motif chemokine 5(CXCL5), is a small cytokine belonging to the CXC chemokine family. It is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon, IFN-γ. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. Full length CXCL5 (78 aa) is trimmed at the N­terminal end by cathepsin G and chymotrypsin to ENA-74 (74 aa) and ENA-70 (70aa), with the shortened forms showing increased potency relative to full length CXCL5. CXCL5can signal through the CXCR2 receptor.Recombinant human ENA-78/CXCL5 (5-78a.a.) produced in E.coli is a single non-glycosylated polypeptide chain containing 74 amino acids. A fully biologically active molecule, rh ENA-78/CXCL5 (5-78a.a.) has a molecular mass of 8 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.

Images & Validation

• Application Notes:
  Reconstituted in ddH2O or PBS at 100 μg/ml.

Key Properties

• Source: Escherichia coli.
• Biological Activity: The EC50 value of human ENA-78/CXCL5 (5-78a.a.) on Caˆ2+ mobilization assay in CHO-K1/ Gα15/hCXCR2 cells (human Gα15 and human CXCR2 stably expressed in CHO-K1 cells) is less than 50 ng/ml.
• Molecular Weight: 8 kDa, observed by reducing SDS-PAGE.
• Protein Sequence: AAV LRELRCVCLQ TTQGVHPKMI SNLQVFAIGP QCSKVEVVAS LKNGKEICLD PEAPFLKKVI QKILDGGNKE N
• Purification: > 95% as analyzed by SDS-PAGE
• Purity: > 95% as analyzed by SDS-PAGE
• Endotoxins: < 0.2 EU/μg, determined by LAL method.

Storage & Handling

• Storage: Lyophilized recombinant human ENA-78°CXCL5 (5-78a.a.) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, human ENA-78°CXCL5 (5-78a.a.) should be stable up to 1 week at 4°C or up to 2 months at -20°C.
• Form/Appearance: Lyophilized after extensive dialysis against PBS.
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

Epithelial Neutrophil Activating Peptide-78, CXCL5