Recombinant TNF alpha (Tumor necrosis factor alpha), Mouse, AF

• Catalog Number: orb1178921
• Category: Proteins
• Description: Recombinant TNF alpha (Tumor necrosis factor alpha), Mouse, AF
• Tag: His-tag at the N-terminus
• Reactivity: Mouse
• Form/Appearance: Lyophilized
• Buffer/Preservatives: The protein was lyophilized from a solution containing 1X PBS, pH 8.0.
• Purity: > 98% as determined by SDS-PAGE. Ni-NTA chromatography.
• Protein Sequence: MLRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQLSAEVNLPKYL DFAESGQVYFGVIAL with polyhistidine tag at the C-terminus.
• Tested applications: ELISA
• Endotoxins: < 0.1 EU per 1 μg of the protein by the LAL method.
• Source: Escherichia coli
• Biological Activity: Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED50 for this effect is < 40 pg/mL. The specific activity of recombinant mouse TNF alpha is approximately > 2.5x 107 IU/mg.
• Storage: Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.
• Alternative names: TNFSF2, Cachectin, Differentiation-inducing factor
• Note: For research use only
• Entrez: 21926